Pengaruh Waktu Penambahan Lactobacillus rhamnosus SKG 34 terhadap Karakteristik Tape Ketan Probiotik Selama Penyimpanan

Authors

  • Emilya Grasiana Dede
  • Komang Ayu Nocianitri Fakultas Teknologi Pertanian, Universitas Udayana
  • Luh Putu Trisna Darmayanti Fakultas Teknologi Pertanian, Universitas Udayana

DOI:

https://doi.org/10.24843/JITPA.2018.v03.i01.p02

Keywords:

karakteristik, Lactobacillus rhamnosus SKG 34, Tape Ketan

Abstract

Tape is one of Indonesia's traditional fermented food that has a sweet and slightly sour taste. The basic ingredients of tape are materials that contain carbohydrates such as cassava and sticky rice. Lactobacillus rhamnosus SKG 34 is one type of lactic acid bacteria isolated from Sumbawa wild horse milk. This study aims to determine the best time addition of Lactobacillus rhamnosus SKG 34 and to determine the effect of the addition of Lactobacillus rhamnosus SKG 34 on the characteristic of probiotics Tape Ketan during storage. The study was conducted in two parts, namely first parts which aimed to know the difference of probiotic addition of Lactobacillus rhamnosus SKG 34 before fermentation and after fermentation. Second research was aimed to find out the viability of Lactobacillus rhamnosus SKG 34 in probiotic tape Ketan and characteristics of tape ketan during storage using Completely Randomized Design with the treatment of storage consisting of 5 levels were day 0, day 1, day 2, day 3 and day 4. The variables were LAB total, sugar total, acid total, pH and sensory characteristics. The results of this research showed that the addition of probiotics before fermentation had a higher LAB than after fermentation  (3.5 x 108 cfu /g). Probiotic tape Ketan  still has a good characteristic until third day  storage with characteristic LAB total 9,0 x 108 cfu/g, sugar total  8,17%, acid total 0,76%, pH 3,54 and color (rather like), Flavor (rather like), texture (rather like), sense hedonic test (rather like), sense scoring test (neutral) and overall acceptance (rather like).

References

Apriyantono, A., D. Fardiaz., N. L. Puspitasari., Sedarnawati., dan S. Budiyanto. 1989. Petunjuk Laboratorium Analisis Pangan. IPB Press. Bogor.

AOAC. 1995. Official Methods of Analysis. Fifteenth Edition. Published by the AOAC, Inc., Suite 400, 2200 Wilson Boeluvard, Arlington, Virginia 2200, USA.

Buckle, K.A., R.A. Edwards, G.H. Fleet, M. Wootton. 1987. Ilmu Pangan. Universitas Indonesia. Jakarta.

Fardiaz, S. 1993. Analisis Mirobiologi Pangan. Raja Grafindo Persada, Jakarta

Hidayat, N., M. C Padasa dan S. Suhartini. 2006. Mikrobiologi Industri. Andi. Yogyakarta.

Jonsen. 1984. Mempelajari Penyimpanan Tape Ubi Kayu (Manihot sp) Sebagai Bahan Mentah Untuk Industri. Skripsi. Fakultas Teknologi Pertanian. IPB. Bogor

Pakpahan, M., C.N Ekowati, dan K. Handayani. Karakterisasi Fisiologi dan Pertumbuhan Isolat Bakteri Bacillus thuringiensis dari Tanah Naungan Di Lingkungan Universitas Lampung. Seminar Nasional Sains & Teknologi V Lembaga Penelitian Universitas Lampung

Martini, T. 2002. Kajian Pembuatan Tepung Cake Tape Ubi Kayu (Manihot esculenta Crantz) Instan Dan Penerimaan Konsumen Terhadap Mutu Organoleptik Cake. Skripsi. Fakultas Teknologi Pertanian. IPB, Bogor.

Nocianitri, K.A., N. S Antara, I.M Sugitha., I.D.M Sukrama., Y.Ramona dan I.N Sujaya. 2017. The effect of two Lactobacillus rhamnosus strains on the blood lipid profile of rats fed with high fat containing diet. International Food Research Journal 24(2): 795-802.

Putri, Y.N. 2007. Mempelajari Pengaruh Penyimpanan Tape Ketan (Oryza sativa glutinosa) Terhadap Daya Terima Konsumen. Skripsi. Fakultas Teknologi Pertanian. Institut Pertanian Bogor.

Purwardhani, S.N., E.S Rahayu, dan M. Suladra, 2008. Efektivitas Suplementasi Agensia Probiotik Lactobacillus acidophilus SNP-2 Pada Pembuatan Tape Ketan dan Brem. Jurnal Agritech Vol. 8

Rahayu, E.S dan S.N Purwandhani. 2004. Suplementasi Lactobacillus achidophilus SNP-2 Pada Tape dan Pengaruhnya Pada Relawan. Jurnal. Teknol dan Industri Pangan, Vol XV, No. 2.

Sastrosupadi, A., 2000. Rancangan Percobaan Praktis Bidang Pertanian. Penerbit Kanisus. Yogyakarta.

Salminem S, C Bouley, MC Boutron-Ruault, Cumings JH, Franck A, Gibson GR, Isolauri E, Moreau MC,

Roberfroid M, and Rowland I. 1998. Functional food science and gastrointestinal physiology and function. Br J Nutr, 80 (suppl) : S147-71.

Steinkraus, K. 1996. Handbook of Indigenous Fermented Foods, Second Edition, Revised and Expanded. CRC Press. New York

Supriyantono. 1995. Mikroorganisme Dalam Ragi Untuk Fermentasi Tape. Di dalam:Prosiding Biomassa, BPPT, Jakarta.

Suryani, Yoni, AB. Oktavia dan S. Umniyati. 2010. Uji Viabilitas Lactobacillus sp. Mar 8 Terenkapsulasi. Bidang Mikrobiologi, Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI), Bogor.

Sujaya, I.N., N.M.U Dwipayanti, N.L.P Suriani., N.P Widarini., K.A, Nocianitri., dan N.W Nursini. 2008a. Potensi Lactobacillus spp. Isolat Susu Kuda Sumbawa sebagai Probiotik. Jurnal Veteriner 9 (2) : 52-59.

Sujaya, I.N., Y, Ramona., N.P, Widarini., N.P Suariani., N.M.U, Dwipayanti., K.A Nocianitri., dan N.W, Nursini. 2008b. Isolasi dan Karakterisasi Bakteri Asam Laktat dari Susu Kuda Sumbawa. Jurnal Veteriner 9 (2) : 52-59.

Agrotechno

Downloads

Published

2018-04-30

How to Cite

Dede, E. G., Nocianitri, K. A., & Darmayanti, L. P. T. (2018). Pengaruh Waktu Penambahan Lactobacillus rhamnosus SKG 34 terhadap Karakteristik Tape Ketan Probiotik Selama Penyimpanan. Jurnal Ilmiah Teknologi Pertanian Agrotechno, 3(1), 269–275. https://doi.org/10.24843/JITPA.2018.v03.i01.p02

Issue

Section

Articles

Similar Articles

<< < 1 2 3 4 5 6 > >> 

You may also start an advanced similarity search for this article.