Isolation and Antibiotic Resistance Profile of Klebsiella spp. from the Gastrointestinal Tract of Broiler Chickens in Poultry Farms in Bali

Authors

  • Yeocelin Meida Utami Udayana University
  • Putu Henrywaesa Sudipa Udayana University
  • I Gusti Ketut Suarjana Udayana University
  • I Nengah Kerta Besung Udayana University
  • Hapsari Mahatmi Udayana University
  • I Gede Putu Anggita Raka Saputra Udayana University
  • Arine Rayka Khaniya Udayana University

DOI:

https://doi.org/10.24843/ATBES.2025.v09.i02.p02

Keywords:

Antimicrobial resistance, Bali, Broiler Chickens, Multidrug ressistant, Klebsiella spp.

Abstract

The poultry sector in Bali has experienced substantial growth, accompanied by a notable increase in broiler chicken populations. Information on Klebsiella spp. outbreaks in Bali has not yet been studied, despite the many potential infectious diseases that may affect broiler chickens on a large scale. Klebsiella spp. is an opportunistic pathogen that can induce severe respiratory disease in poultry, carrying a potential risk of zoonotic transmission. Furthermore, the increasing incidence of antibiotic resistance among Klebsiella strains poses a critical challenge to effective therapeutic interventions. This study was conducted to isolate and identify Klebsiella spp. from the gastrointestinal tract of broiler chickens in commercial farms across Bali, and to assess the antimicrobial resistance profiles of the isolates against multiple antibiotic agents. A cross-sectional study with a total of 25 cloacal swabs was conducted, and the swabs were subjected to microbiological analysis. Isolation was performed using selective MacConkey Agar (MCA), followed by Gram staining, biochemical identification, and antibiotic susceptibility testing through the Kirby-Bauer disk diffusion method. Two out of 25 (8%) samples were positively identified as Klebsiella spp. All of these positive isolates showed complete resistance to ampicillin, sulfamethoxazole, erythromycin, ciprofloxacin, and tetracycline. Additionally, the positive isolates also exhibited intermediate resistance against kanamycin, while 50% of the isolates demonstrated susceptibility to chloramphenicol. These findings confirm the presence of multidrug-resistant (MDR) strains among the isolates examined. The outcomes of this research emphasize the urgent need for a rigorous study to identify potential outbreaks caused by Klebsiella spp., throughout commercial farms in Bali and stringent monitoring and regulation of antibiotic usage within the poultry industry to mitigate the proliferation of antimicrobial resistance, thereby safeguarding animal health and preventing potential risks to public health.

Author Biographies

Yeocelin Meida Utami, Udayana University

Department of Bacteriology and Mycology, Faculty of Veterinary Medicine

Putu Henrywaesa Sudipa, Udayana University

Department of Bacteriology and Mycology, Faculty of Veterinary Medicine

I Gusti Ketut Suarjana, Udayana University

Department of Bacteriology and Mycology, Faculty of Veterinary Medicine

I Nengah Kerta Besung, Udayana University

Department of Bacteriology and Mycology, Faculty of Veterinary Medicine

Hapsari Mahatmi, Udayana University

Department of Bacteriology and Mycology, Faculty of Veterinary Medicine

I Gede Putu Anggita Raka Saputra, Udayana University

Undergraduate Student of Veterinary Medicine, Faculty of Veterinary Medicine

Arine Rayka Khaniya, Udayana University

Undergraduate Student of Veterinary Medicine, Faculty of Veterinary Medicine

Published

2025-06-30

How to Cite

Utami, Y. M., Sudipa, P. H., Suarjana, I. G. K., Besung, I. N. K., Mahatmi, H., Saputra, I. G. P. A. R., & Khaniya, A. R. (2025). Isolation and Antibiotic Resistance Profile of Klebsiella spp. from the Gastrointestinal Tract of Broiler Chickens in Poultry Farms in Bali. Advances in Tropical Biodiversity and Environmental Sciences, 9(2). https://doi.org/10.24843/ATBES.2025.v09.i02.p02