Pengaruh Media Fermentasi Terhadap Produksi dan Karakterisasi Selulosa Bakteri Sari Pelepah Kelapa Sawit
Pengaruh Media Fermentasi Terhadap Produksi dan Karakterisasi Selulosa Bakteri Sari Pelepah Kelapa Sawit
DOI:
https://doi.org/10.24843/JITPA.2026.v11.i01.p05Abstract
Fresh oil palm frond juice with mechanical pressing contains a concentration of easily fermented sugars such as glucose, sucrose, and fructose, suitable for use as a fermentation raw material because it does not inhibit microbial growth does not pose a risk to health and safety. The purpose of this study was to determine the effect of fermentation medium pH on bacterial cellulose from oil palm frond juice. Bacterial cellulose was produced using Acotobacter xilynum. Oil palm frond juice was prepared at 100 ml/g with the addition of protein with different pH media fermented for 8 days at room temperature in statistical conditions. The results of the thickness and weight of the best cellulose bacteria of pH 5 medium were 0.78 cm and 393,015 g/L. FE-SEM micrograph analysis for bacterial cellulose showed typical peaks found in cellulose with typical fibril threads. pH 3 had the lowest crystallinity value and the best crystallinity was at pH 5 based on FWHM. 1106 and 1423 cm-1 wave number is the C-O-C or C-O bond areas which are one of the bonds of the main chain of bacterial cellulose and the best tensile strength is the pH 5 treatment with a strength of 261.645 Mpa
References
Asthary, P. B., Saepulloh, S., Sanningtyas, A., Pertiwi, G. A., Purwita, C. A., & Septiningrum, K. (2021). Optimasi Produksi Bacterial Nanocellulose dengan Metode Kultur Agitasi. Jurnal Selulosa, 10(02), 89. https://doi.org/10.25269/jsel.v10i02.295
Athirah Raihana Nor Aziz Hashim, N., Zakaria, J., Mohamad, S., Fathiyah Sy Mohamad, S., & Ab. Rahim, M. H. (2021). Effect of Different Treatment Methods on the Purification of Bacterial Cellulose Produced from OPF Juice by Acetobacter Xylinum. IOP Conference Series: Materials Science and Engineering, 1092(1), 012058. https://doi.org/10.1088/1757-899x/1092/1/012058
Baghaei, B., & Skrifvars, M. (2020). All-Cellulose Composites: A Review of Recent Studies on Structure, Properties and Applications. Molecules, 25(12). https://doi.org/10.3390/molecules25122836
Cheng, K., Catchmark, J. M., & Demirci, A. (2009). Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis. Journal of Biological Engineering, 10, 1–10. https://doi.org/10.1186/1754-1611-3-12
Daicho, K., Saito, T., Fujisawa, S., & Isogai, A. (2018). The Crystallinity of Nanocellulose: Dispersion-Induced Disordering of the Grain Boundary in Biologically Structured Cellulose. ACS Applied Nano Materials, 1(1), 5744–5785. https://doi.org/10.1021/acsanm.8b01438
Faisul Aris, F. A., Mohd Fauzi, F. N. A., Tong, W. Y., & Syed Abdullah, S. S. (2019). Interaction of silver sulfadiazine wıth bacterial cellulose via ex-situ modification method as an alternative diabetic wound healing. Biocatalysis and Agricultural Biotechnology, 21(September), 101332. https://doi.org/10.1016/j.bcab.2019.101332
Fijałkowski, K., Rakoczy, R., Zywicka, A., Drozd, R., Zielińska, B., Wenelska, K., Cendrowski, K., Peitler, D., Kordas, M., Konopacki, M., & Mijowska, E. (2016). Time dependent influence of rotating magnetic field on bacterial cellulose. International Journal of Polymer Science, 2016. https://doi.org/10.1155/2016/7536397
Fuller, M. E., Andaya, C., Mcclay, K., Services, F., Fuller, M. E., & Services, A. F. (2017). Evaluation of ATR-FTIR for analysis of bacterial cellulose impurities. Jurnal Biocellulose Impurity Analysis, 1–19.
Gündüz, G., & Aşık, N. (2018). Production and characterization of bacterial cellulose with different nutrient source and surface–Volume ratios. Drvna Industrija, 69(2), 141–147. https://doi.org/10.5552/drind.2018.1744
Harrison, T. R., Kumar, V., Alam, P., Willis, A., Scarpa, F., & Kumar, V. (2023). From trash to treasure : Sourcing high-value , sustainable cellulosic materials from living bioreactor waste streams. International Journal of Biological Macromolecules, 233(February), 123511. https://doi.org/10.1016/j.ijbiomac.2023.123511
Hossain, M. A., Hoque, M. M., Hossain, M. M., Kabir, M. H., Yasin, M., & Islam, M. A. (2020). Biochemical , Microbiological and Organoleptic Properties of Probiotic Pineapple Juice Developed by Lactic Acid Bacteria.
Irzaman, Y, S., M, H., W, L., & Barmawi M. (2000). Analisis Struktur Kristal Dan Full Width Half Maximum (Fwhm) Dengan Metode Rietveld (Studi Kasus : Kalsit (Caco3)). Jurnal Kontribusi Fisika Indonesia., 11(2), 41–48.
Kasim, N., & Rahman, N. A. (2016). Design and production control of biocellulose from Acetobacter xylinum. Indian Journal of Science and Technology, 9(21). https://doi.org/10.17485/ijst/2016/v9i21/95241
Kuo, C. H., Chen, J. H., Liou, B. K., & Lee, C. K. (2016). Utilization of acetate buffer to improve bacterial cellulose production by Gluconacetobacter xylinus. Food Hydrocolloids, 53, 98–103. https://doi.org/10.1016/J.FOODHYD.2014.12.034
Mohamad, S., Abdullah, L. C., Jamari, S. S., Osman Al Edrus, S. S., Aung, M. M., & Sy Mohamad, S. F. (2022). Production and Characterization of Bacterial Cellulose Nanofiber by Acetobacter Xylinum 0416 Using Only Oil Palm Frond Juice as Fermentation Medium. Journal of Natural Fibers, 19(17), 16005–16016. https://doi.org/10.1080/15440478.2022.2140243
Nimitkeatkai, H., Fong-In, S., & Potaros, T. (2020). Characterization of Bacterial Cellulose (Nata de coco) from Lychee. IOP Conference Series: Earth and Environmental Science, 515(1). https://doi.org/10.1088/1755-1315/515/1/012063
Ramli, U. S., Tahir, N. I., Rozali, N. L., Othman, A., Muhammad, N. H., Muhammad, S. A., Ahmad Tarmizi, A. H., Hashim, N., Sambanthamurthi, R., Singh, R., Abd Manaf, M. A., & Ahmad Parveez, G. K. (2020). Sustainable palm oil—The role of screening and advanced analytical techniques for geographical traceability and authenticity verification. Molecules, 25(12), 1–26. https://doi.org/10.3390/molecules25122927
Revin, V., Liyaskina, E., Nazarkina, M., Bogatyreva, A., & Shchankin, M. (2018). Cost-effective production of bacterial cellulose using acidic food industry by-products. Brazilian Journal of Microbiology, 49, 151–159. https://doi.org/10.1016/j.bjm.2017.12.012
Said Azmi, S. N. N., Mohd Fabli, S. N. N. F., Faisul Aris, F. A., Samsu, Z. A., Mohd Asnawi, A. S. F., Mohamed Yusof, Y., Ariffin, H., & Syed Abdullah, S. S. (2019). Fresh oil palm frond juice as a novel and alternative fermentation medium for bacterial cellulose production. Materials Today: Proceedings, 42(xxxx), 101–106. https://doi.org/10.1016/j.matpr.2020.10.220
Sharifah Fathiyah, S. M., Shahril, M., & Junaidi, Z. (2021). Oil palm frond juice and coconut water as alternative fermentation substrate for bacterial cellulose production. IOP Conference Series: Materials Science and Engineering, 1092(1), 012055. https://doi.org/10.1088/1757-899x/1092/1/012055
Sou Min, T. (2023). Bacterial Cellulose Production From Oil Palm Frond Juice and Its Impregnation With Silver Nanoparticles for Antibacterial Wound Dressing. Journal of Oil Palm Research. https://doi.org/10.21894/jopr.2023.0055
Supian, N. N. I., Zakaria, J., Amin, K. N. M., Mohamad, S., & Mohamad, S. F. S. (2021). Effect of fermentation period on bacterial cellulose production from oil palm frond (OPF) juice. IOP Conference Series: Materials Science and Engineering, 1092(1), 012048. https://doi.org/10.1088/1757-899x/1092/1/012048
Tyagi, N., & Suresh, S. (2016). Production of cellulose from sugarcane molasses using Gluconacetobacter intermedius SNT-1: Optimization & characterization. Journal of Cleaner Production, 112(January), 71–80. https://doi.org/10.1016/j.jclepro.2015.07.054
Wang, J., Tavakoli, J., & Tang, Y. (2019). Bacterial cellulose production, properties and applications with different culture methods – A review. Carbohydrate Polymers, 219(May), 63–76. https://doi.org/10.1016/j.carbpol.2019.05.008
Ye, J., Zheng, S., Zhang, Z., Yang, F., Ma, K., Feng, Y., Zheng, J., Mao, D., & Yang, X. (2019). Bacterial cellulose production by Acetobacter xylinum ATCC 23767 using tobacco waste extract as culture medium. Bioresource Technology, 274(December 2018), 518–524. https://doi.org/10.1016/j.biortech.2018.12.028
Zahari, M. A. K. M., Zakaria, M. R., Ariffin, H., Mokhtar, M. N., Salihon, J., Shirai, Y., & Hassan, M. A. (2012). Renewable sugars from oil palm frond juice as an alternative novel fermentation feedstock for value-added products. Bioresource Technology, 110, 566–571.https://doi.org/10.1016/j.biortech.2012.01.119
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Arief Fazlul Rahman, Adiba Iffadhya Fathin, Nasution Fachri Ibrahim

This work is licensed under a Creative Commons Attribution 4.0 International License.