The Effect Of Amylase Enzyme Concentration And Hydrolysis Time Of Rubber Singkong Rubber (Manihot Glaziovii Muell. Arg) Rubber Starch On Total Reducing Sugar Produced

Abstract

Cassava is one of the food substitutes for rice which has an important role in supporting the food security  of a region. One of the tubers that has the potential to be used as raw material for making simple sugars  is rubber cassava tubers. Carbohydrate content contained in rubber cassava is 98.4674% of the total  dry weight of cassava so that rubber cassava has great potential to be used as a source of starch  manufacture. This study will determine the effect of -amylase enzyme concentration and hydrolysis time  of rubber cassava crude starch on the total reducing sugar produced. In addition, to obtain the  concentration of -amylase enzyme and hydrolysis time of rubber cassava crude starch which can  produce the highest total reducing sugar. This research was conducted by optimizing the amylase  enzyme concentration (0.4 U/g, 0.8 U/g, 1.2 U/g) and the length of time (60, 120, 180 minutes) in  hydrolysis. The results obtained in this study, namely the concentration of enzymes and duration of  hydrolysis affect the total sugar produced. The best treatment was obtained in the K2H3 treatment  (enzyme concentration 0.8 U/g and hydrolysis duration 120 minutes) with a total value of reducing  sugar 2.667 mg/mL and a final pH of 5.30 and a final TPT of 32% Brix.