Developmental Study Of Soxhletation Extraction Methods On The Antioxidant Acidity Of Matoa (Pomitea Pinnata) Leaves Extract Using Uv Vis Spectrofotometer

Abstract

This study aims to examine the development of extraction methods for antioxidant capacity testing using a spectrophotometer on matoa leaf extract. The extraction method used is the soxhletation method. Factors that affect the extraction process with Soxhlet tools include extraction time and solvent concentration. The treatments used in this study were ethanol concentration (60%, 70%, 80% and 90%) and extraction time (3, 4, 5, and 6 hours), Determination of the best solvent concentration and extraction time was carried out by calculating the antioxidant content of the DPPH-spectrophotometer method. The results showed that 70% ethanol concentration with extraction time for 4 hours was the best treatment that obtained antioxidant capacity of 5.54% with extract yield of 35.00%. The antioxidant capacity of matoa leaf extract obtained ranged from 3.15-5.54% with the yield value of matoa leaf extract obtained ranged from 19.36 - 35.00% which has color characteristics, namely the range of L* values of 13.39 to 20.44, the range of a* values ranging from -3.62 to 0.43 and the range of b* values ranging from -7.50 to -3.31. The soxhletation method can be used as one of the extraction methods to obtain matoa leaf extract and the use of UV-VIS spectrophotometric method can also be used as one of the methods for testing the antioxidant capacity of matoa leaf extract.