Effect Of Stirring Speed And Duration Of Microemulsion Characteristics Of Clove Essential Oil (Syzygium Aromaticum)

Abstract

The main product of the clove plant (Syzygium aromaticum) is essential oil. Clove oil has the advantage of being able to inhibit the growth of Staphylococcus aureus compared to other essential oils containing eugenol. The nature of essential oils is volatile so that in this study clove oil was made in the form of microemulsions because microemulsions are thermodynamically stable, transparent and have low viscosity. The characteristics of the microemulsion are influenced by several factors including the duration of stirring and the speed of stirring. The purpose of this study was to determine the effect of speed and duration of stirring on the characteristics of clove essential oil microemulsions and to determine the best speed and duration of stirring to produce the characteristics of clove essential oil microemulsions. This experiment used a randomized block design with two factors, namely speed and duration of stirring. Stirring was carried out for 4, 6 and 8 minutes and the stirring speed was 600, 700, 800 and 900 rpm. Data were analyzed for variance using excel, if there was an effect of treatment on the observed variables, the analysis was continued with the BNJ Test. The results showed that the combination of speed and duration of stirring had an effect on the characteristics of the resulting microemulsion. A speed of 600 rpm and a stirring time of 4 minutes was the best treatment for making clove essential oil microemulsions with the smallest turbidity index values ​​after 24 hours of incubation and after centrifugation, namely 0.449 ± 0.026% and 0.473 ± 0.047%. Clove essential oil microemulsion particle size was 11.3 ± 2.5 nm. Clove essential oil microemulsion was stable for 8 weeks of storage.