Effectiveness Of Disinfectant Bleach Solution (Bayclin) In Preventing Microbial Contaminants In Incubators In The Microbiology Laboratory

Abstract

Contaminants in microbiological analysis often occur even though aseptic testing has been carried out. Control of the growth of microorganisms aims to eradicate unwanted microorganisms. The disinfectant used is a combination of bleach solution (bayclin) and alcohol which works by inactivating enzymes, denaturing proteins and inactivating nucleic acids. The purpose of this study was to determine the effectiveness of using a disinfectant bleach solution (bayclin) of various concentrations in preventing microbial contaminants in the incubator at the Food Microbiology Laboratory. The data collection technique in this study was in the form of an experiment, the results of surface swabs of incubator equipment that had been sprayed with 70% alcohol followed by spraying a disinfectant bleach solution (bayclin) with concentrations (0%, 5%, 10% and 15%), then testing the level of microbial contaminants by analysis of total bacteria and total yeast molds using the TPC (Total Plate Count) method with 3 repetitions. It was found that spraying disinfectant bleach solution (bayclin) had an effect on the total quantity of bacteria and total yeast mold incubated in the incubator equipment in the microbiology laboratory with the highest total bacterial cell quantity in the control treatment (bayclin 0%) of 3.7 CFU/cm2 and the smallest by 0 CFU/cm2 in the treatment of 15% bayclin concentration and the highest cell quantity of total yeast in the control treatment (0% bayclin) was 1.5 CFU/cm2 and the smallest was 0 CFU/cm2 in the 15% bayclin concentration treatment.