Determination Of Fermentation Time And Initial Ph Of Media In Ethanol Production By Isolate Bm1-Cp14

Abstract

This study aims to determine the optimum duration of fermentation and the best initial pH of the growth  medium BM1-CP14 isolates to produce ethanol. In this study, five treatments of fermentation time (2, 4,  6, 8 and 10 days) combined with three initial pH treatments of the fermentation medium (4, 5, and 6)  were conducted. This research was carried out in several steps, namely cell rejuvenation, propagation  of BM1-CP14 isolates, fermentation, distillation, and calculation of total ethanol. The best fermentation  duration was found to be ten days with a difference of total dissolved solid 1.07 (∆%brix) yielded total  ethanol of 5.08 mL. In the initial pH treatment, the media produced total ethanol at pH 4 of 5.15 mL,  pH 5 of 5.17 mL and pH 6 of 5.16 mL. The pH treatment of BM1-CP14 bacteria was found to have no  effect due to the total ethanol produced was almost similar